When laser welding is directly used for steel/aluminum joints, brittle Fe-Al intermetallic compounds are easily produced. For the issue, the effects of silver interlayer on microstructure and mechanical properties of 304 stainless steel/6061 aluminum alloy welded joint were investigated by utilizing double laser weldi ng in the paper. The results of the research showed that the interlayer was the prefabricated by silver, and the joint was formed well by dual laser welding process. Organizational analysis showed that the weld area was obviously divided into area A near the aluminum side and area B near the steel side. Area A was a silver-rich phase mixed with aluminum-rich phase, and area B was made from pure silver zone（B1） and austenitic zone（B2）. In addition, Fe-Al brittle intermetallic compounds didn’t appear in the weld seam. The results of mechanical properties showed that the fracture occurred in the pure silver zone of the joint, and the tensile strength was175.59 MPa, and the elongation after fracture was about 3%.