The electron beam fuse additive manufacturing of GH4169 multi-layer multi-pass deposition sample block was studied,and the microstructure and mechanical properties of the deposition block of electron beam fuse additive manufacturing at different heights and different forming directions were analyzed. The results show that there is obvious anisotropy in the microstructure and mechanical properties of GH4169 additive manufactured by electron beam fuse. The microstructures of the formed samples are mainly γ phase,eutectic γ +Laves phase and carbide phase. There are a large number of Laves bands between dendrites;the microstructure is different in different forming directions,the microstructure along the wire feed direction（Ds） is isometric "cross-shaped" γ dendrites,the typical microstructure of the deposition height direction（Dd）and horizontal direction（ Dt） is columnar crystals. The EDS results show that a small amount of Nb and Mo elements segregated in the same layer. Along the deposition height direction,the microhardness gradually shows an upward trend,and along the horizontal direction,the microhardness basically fluctuates around a certain value. The tensile proper-ties are obviously anisotropic,and the tensile strength along the wire feed direction（Ds） and horizontal direction（Dt） is signifi-cantly higher than that along the deposition height direction（Dd）,and the overall tensile strength is lower than that of high temperature alloy forgings at room temperature.